Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Forum > Chit Chat
Register Search Today's Posts Mark Forums Read

rna cleanup problems

rna cleanup problems - Chit Chat

rna cleanup problems -

LinkBack Thread Tools Display Modes
Old 12-12-2012, 01:11 PM
Pipette Filler
Points: 22, Level: 1 Points: 22, Level: 1 Points: 22, Level: 1
Activity: 0% Activity: 0% Activity: 0%
Join Date: Dec 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Thumbs down rna cleanup problems

hej everybody,
i have extracted total rna from 24 well plate mono layer cultures by using trizol method. the amount isolated is good but the quality 260/280 is between 1.4 to 1.7 only but the 260/230 ratio is less than 0.5. so i tried to clean up the rna by using qiagen rneasy mini and rneasy microkit and zymoresearch's rna clean and concentrator. after cleaning i am unable to see the rna and the nanodrop is showing negative results indicating absence of rna. all the three methods have shown the same. i am sure that rna is present in the sample because before cleaning i again checked the rna values in nanodrop. could anyone suggest me what is the problem and how to rectify and how to remove the protein contamination as indicated by 260/230 ratio.
thanks in advance.
Reply With Quote

cleanup , problems , rna , rna cleanup

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Plasmid-stability? Fragment-deletion? I'm in dire need to identify and solve problems TGS Molecular Cloning Forum 3 10-22-2012 09:17 PM
Research News - Infants with persistent crying problems more likely to have behavior moleculardude Science News and Views 0 04-21-2011 11:14 AM
problems with growing healthy Arabidopsis plants Monika Kuzma Arabidopsis and Plant Biology 1 02-19-2007 03:55 PM
Problems with growing healthy Arabidopsis plants - light intesity? Karl Lundy Arabidopsis and Plant Biology 0 02-07-2007 05:11 PM

All times are GMT. The time now is 11:18 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11303 seconds with 16 queries