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Old 10-01-2012, 01:35 AM
Pipette Filler
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Default epigenetic switch



Dear all,
This was one of the questions of my last exam, anybody has any Idea on that?

An unknown prokaryotic organism is carries a CRO-GFP transcriptional fusion on a lambda pro-phage that lacks any other lambda genes except cI. In this organism, cI and cro are expressed normally and hence the CRO-GFP fusion is driven by the lambda PR promoter. This strain exhibits a GFP- phenotype when the cI is expressed. But temporary RecA-assisted inactivation of the CI repressor results in a heritable GFP+ (epigenetic) phenotype. Why? and How?
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