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His tag purification buffers

His tag purification buffers - Chit Chat

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Old 05-30-2009, 06:17 PM
Pipette Filler
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Unhappy His tag purification buffers



Hi I am attempting to purifiy a his tagged protein...I would like to know whether i should use sodium phosphate monobasic or dibasic in my my buffers.The recipie just says sodium phosphate? is there a difference between the two when using them in my binding and elution buffers..thanks..
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Old 06-04-2009, 05:57 AM
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Default Re: His tag purification buffers

phosphate monobasic
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Old 06-05-2009, 01:54 AM
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Default Re: His tag purification buffers

Quote:
Originally Posted by lateralus_71 View Post
Hi I am attempting to purifiy a his tagged protein...I would like to know whether i should use sodium phosphate monobasic or dibasic in my my buffers.The recipie just says sodium phosphate? is there a difference between the two when using them in my binding and elution buffers..thanks..

Hi!
I work with recombinant proteins too.
The only one difference between the two is the pH that you'll have to adjust.
Just it!
I already used the both and I saw no difference or any trouble in my experiments and results.

Good luck!
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