| |||||||
| Register | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
 |
| | LinkBack | Thread Tools | Display Modes |
05-30-2009, 07:17 PM
| |||||||||||
| |||||||||||
 His tag purification buffers Hi I am attempting to purifiy a his tagged protein...I would like to know whether i should use sodium phosphate monobasic or dibasic in my my buffers.The recipie just says sodium phosphate? is there a difference between the two when using them in my binding and elution buffers..thanks..  |
|
06-04-2009, 06:57 AM
| |||||||||||
| |||||||||||
 Re: His tag purification buffers phosphate monobasic |
|
06-05-2009, 02:54 AM
| |||||||||||
| |||||||||||
| Re: His tag purification buffers Quote:
Hi! I work with recombinant proteins too. The only one difference between the two is the pH that you'll have to adjust. Just it! I already used the both and I saw no difference or any trouble in my experiments and results. Good luck! |
|
| Tags |
| buffers , purification , tag |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| His-Tag purification - contaminated protein | Elayoe | Recombinant Protein Forum | 3 | 09-29-2009 10:10 PM |
| His-tag protein purification under denaturing conditions | Arabidop | Molecular Biology Articles and Protocols | 5 | 06-28-2009 07:05 AM |
| inactive enzyme after purification with Ni-TED | piefke | Recombinant Protein Forum | 0 | 05-02-2008 09:14 AM |
| Losing DNA in purification? | cheetara_2001 | DNA Techniques | 4 | 05-28-2007 08:11 PM |
| Electrophoresis Buffers - Unlimited Lifespan? | Wolfgang Schechinger | Protocols and Methods Forum | 2 | 12-28-2005 09:59 AM |
Linear Mode
