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ChIP Chromatin Immunoprecipitation Forum Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


reagents for ChIP

reagents for ChIP - ChIP Chromatin Immunoprecipitation Forum

reagents for ChIP - Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


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  #1  
Old 01-04-2013, 08:56 PM
Pipette Filler
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Default reagents for ChIP



Hi, I tried many times but I still cannot get a good sonication condition for my exp. Now I used all my lysis buffer which I bought from Millipore(EZ-Magna ChIP™ A Chromatin Immunoprecipitation Kit). I checked their web but no recipe for it.There are so many different recipes for lysis buffer. I do not know which one is good for my exp.

So is there someone who could give me suggestions to make my own cell lysis buffer and nuclear lysis buffer?

Thanks a lot!
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  #2  
Old 01-24-2013, 09:44 AM
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Default Re: reagents for ChIP

As far as I know, you can get different sonicated conditions using a lysis buffer with or without SDS. I don't know anything about Millipore kit...
Also, when you are sonicating, take care about bubbles, because bubbles decrease the eficiency of sonication... Which is the brand of your sonicator? Try to change some parameters, like amplitude, times, etc.
To make your own buffer, first we need to know what kind of material are you working with.

I hope it has been useful!
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  #3  
Old 02-13-2013, 08:31 AM
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