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-   -   reagents for ChIP (http://www.molecularstation.com/forum/chip-chromatin-immunoprecipitation-forum/87275-reagents-chip.html)

ChIP Kick 01-04-2013 08:56 PM

reagents for ChIP
 
Hi, I tried many times but I still cannot get a good sonication condition for my exp. Now I used all my lysis buffer which I bought from Millipore(EZ-Magna ChIP A Chromatin Immunoprecipitation Kit). I checked their web but no recipe for it.There are so many different recipes for lysis buffer. I do not know which one is good for my exp.

So is there someone who could give me suggestions to make my own cell lysis buffer and nuclear lysis buffer?

Thanks a lot!

pluja 01-24-2013 09:44 AM

Re: reagents for ChIP
 
As far as I know, you can get different sonicated conditions using a lysis buffer with or without SDS. I don't know anything about Millipore kit...
Also, when you are sonicating, take care about bubbles, because bubbles decrease the eficiency of sonication... Which is the brand of your sonicator? Try to change some parameters, like amplitude, times, etc.
To make your own buffer, first we need to know what kind of material are you working with.

I hope it has been useful!

bamaximujnry3570 02-13-2013 08:31 AM

Uggブーツ格安
 
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