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-   -   Sonication's fault or gel's fault? (http://www.molecularstation.com/forum/chip-chromatin-immunoprecipitation-forum/86990-sonications-fault-gels-fault.html)

ChIP Kick 12-13-2012 03:49 AM

Sonication's fault or gel's fault?
 
2 Attachment(s)
Hi guys,

I have some problems with my ChIP assay. Hope you can give me some suggestions.

I sheared some formaldehyde cross-linked tissues samples. Then, treated with RNase A at 37C for 30min. After adding protinase K, reversed crosslinking at 62C for 2hours. Then run a 1% agarose gel with 85voltage.

Here are the problems. I do not know why there are red lines(in attached file) in my gel when running? In gel images, why I do not have smeared DNA?

My sonicator is Qsonics Q125. Sonication with 30% power, 15sec on, 45sec off, 10cycyles.

Is there someone can help me? Thank you very much!


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