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ChIP - How long can crosslinked material be stored?

ChIP - How long can crosslinked material be stored? - ChIP Chromatin Immunoprecipitation Forum

ChIP - How long can crosslinked material be stored? - Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


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Old 09-21-2012, 03:49 PM
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Lightbulb ChIP - How long can crosslinked material be stored?



I would like to know once you have crosslinked the material and then it has been frozen in liquid nitrogen, can you store it for a long time in -80C? Would resolution decrease?

Thanks!
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Old 10-05-2012, 03:15 PM
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Default Re: ChIP - How long can crosslinked material be stored?

Quote:
Originally Posted by pluja View Post
I would like to know once you have crosslinked the material and then it has been frozen in liquid nitrogen, can you store it for a long time in -80C? Would resolution decrease?

Thanks!
I think snap frozen material stored at -80c will be stable for a relatively long time. I have stored material like this for several weeks and the ChIP-seq worked nicely
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Old 10-10-2012, 03:18 PM
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Default Re: ChIP - How long can crosslinked material be stored?

Haring et al (2007) says that crosslinked plant material can be stored for several months at -80C after freezing in liquid nitrogen.
Thanks to reply mmorgan!

Haring, M., Offermann, S., Danker, T., Horst, I., Peterhansel, C., & Stam, M. (2007). Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization. Plant methods, 3, 11.
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