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-   -   ChIP - How long can crosslinked material be stored? (http://www.molecularstation.com/forum/chip-chromatin-immunoprecipitation-forum/86495-chip-how-long-can-crosslinked-material-stored.html)

pluja 09-21-2012 03:49 PM

ChIP - How long can crosslinked material be stored?
 
I would like to know once you have crosslinked the material and then it has been frozen in liquid nitrogen, can you store it for a long time in -80C? Would resolution decrease?

Thanks!

mmorgan 10-05-2012 03:15 PM

Re: ChIP - How long can crosslinked material be stored?
 
Quote:

Originally Posted by pluja (Post 442231)
I would like to know once you have crosslinked the material and then it has been frozen in liquid nitrogen, can you store it for a long time in -80C? Would resolution decrease?

Thanks!

I think snap frozen material stored at -80c will be stable for a relatively long time. I have stored material like this for several weeks and the ChIP-seq worked nicely

pluja 10-10-2012 03:18 PM

Re: ChIP - How long can crosslinked material be stored?
 
Haring et al (2007) says that crosslinked plant material can be stored for several months at -80C after freezing in liquid nitrogen.
Thanks to reply mmorgan!

Haring, M., Offermann, S., Danker, T., Horst, I., Peterhansel, C., & Stam, M. (2007). Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization. Plant methods, 3, 11.


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