| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| ChIP Chromatin Immunoprecipitation Forum Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
10-31-2011, 06:59 PM
| |||||||||||
| |||||||||||
 Low DNA yield from neutrophils Hello, I am using the Life Technologies MAGnify ChIP kit with 2 X10^6 freshly isolated human neutrophils. About 50% of the time, I get a decent smear on my agarose gel at the right size, and 50% of the time the smear is very faint. I have optimized the shearing conditions to get a population of fragments of the desired size but am struggling to understand why the yield of DNA is so variable. I cross-link for 10 minutes with 1% formadlehyde and am using the Covaris S2 for 12 minutes shear consistently with my cells. Any guidance about what may affect the yield of DNA would be greatly appreciated.  |
|
| Tags |
| chip , dna , dna yield , low , neutrophils , yield |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Neutrophils activation- HELP | Mailendj | Immunology and Host-Pathogen Interactions | 16 | 02-12-2013 01:51 PM |
| How I have to calculate the theoretical yield of.... | Valine | Chemistry Forum | 2 | 11-02-2011 02:45 AM |
| How to calculate the yield... | Valine | Peptide Forum | 1 | 06-06-2011 10:08 AM |
| Help- aCGH Agilent Arrays | Helena Suarez | Microarrays Forum | 1 | 03-17-2011 06:24 PM |
| AFLP low yield | aria johnson | Protocols and Methods Forum | 1 | 06-03-2009 10:54 AM |
Linear Mode
