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Sonication + enzyme digestion

Sonication + enzyme digestion - ChIP Chromatin Immunoprecipitation Forum

Sonication + enzyme digestion - Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


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Old 11-25-2010, 12:51 PM
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Default Sonication + enzyme digestion



Hello everyone,

I need to add to my sonication step, an enzymatic digestion.

My question is: should I use DNASE I (like in footprint) or Micrococcal nuclease (the most publicated enzyme on ChIP)?

What is the reason for use of Micrococcal Nuclease on ChiP and not DNASE I?

Thanks
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digestion , enzyme , sonication


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