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-   -   Sonication + enzyme digestion (http://www.molecularstation.com/forum/chip-chromatin-immunoprecipitation-forum/74399-sonication-enzyme-digestion.html)

Arastho 11-25-2010 12:51 PM

Sonication + enzyme digestion
 
Hello everyone,

I need to add to my sonication step, an enzymatic digestion.

My question is: should I use DNASE I (like in footprint) or Micrococcal nuclease (the most publicated enzyme on ChIP)?

What is the reason for use of Micrococcal Nuclease on ChiP and not DNASE I?

Thanks


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