Sonication + enzyme digestion
I need to add to my sonication step, an enzymatic digestion.
My question is: should I use DNASE I (like in footprint) or Micrococcal nuclease (the most publicated enzyme on ChIP)?
What is the reason for use of Micrococcal Nuclease on ChiP and not DNASE I?
|All times are GMT. The time now is 01:33 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved