ChIP with 100% confluent cells

[kab2]

Hi all,

I'm using NHEK cells and growing them to 100% confluence because they react to my treatment best that way. The problem is I'm having trouble lysing and homogenizing the cells, they get stuck in clumps and my nuclei yield is very low. It doesn't matter if I homogenize 5 times or 100 times, I still get the same results: low input. Any suggestions on what I should do?

I'm currently using the Active Motif enzymatic ChIP-it express kit.

Thanks in advance for any help

[bidisha78]

You can opt for sonication instead of homogenisation and then maybe use the enzyme kit, I had the same problem but it helped.