I'm not sure what is happening with my procedure so I need your ideas if you please.
I'm working with 25x10^6 293T cells concentratred in 1mL. I need this amount to the ChIP assay.
When I go to sonicate, and I'm testing different conditions, I only get smears higher than 1000bp.
I tested different cells concentration: 12x10^6 and 5x10^6
I'm working in the maximum power p/10 seconds avoiding overheating the samples.
I didnīt test the % duty cicle, but I will...
But anyone have another idea? I need 200bp fragments.
Re: Sonication innefective?
For the 61 views until today and no answers, here is the answer:
Is the formaldehyde %.
I'm using 1%.
I tested 0,2%; 0,4%; 0,6%; 0,8%. With 0,8% i get my 200 bp bands and the CHIP worked.
Hope that this could help anyone.
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