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ChIP Chromatin Immunoprecipitation Forum Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


Strain specificity

Strain specificity - ChIP Chromatin Immunoprecipitation Forum

Strain specificity - Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


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Old 04-29-2009, 05:11 PM
Pipette Filler
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Question Strain specificity



How important is the strain of the animal in ChIP?

If I want to ChIP a transcription factor from chromatin obtained from Sprague-Dawley rat tissue, but detect my enrichment using the published sequence of the Norway rat, is this likely to cause me problems with sequence mismatches in regulatory regions between the two strains?

Has anyone had any experiences like this?
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