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ChIP Chromatin Immunoprecipitation Forum Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


chIP antibodies

chIP antibodies - ChIP Chromatin Immunoprecipitation Forum

chIP antibodies - Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


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  #1  
Old 03-27-2009, 01:23 AM
Pipette Filler
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Default chIP antibodies



What is the best procedure to easily determine if an antibody is suitable for chIP? We are using human cells and an in-house generated affinity purified polyclonal antibody that is OK on westerns.
thanks, DC
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Old 04-29-2009, 05:16 PM
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Default Re: chIP antibodies

There are numerous ways people have tested to see if their antibody might be suitable for ChIP, western blot, IHC...in my experience there is no way to know for sure until you actually try it in the a ChIP protocol. If you don't already have a ChIP protocol in the lab for other antibodies then get yourself a quick kit with a suitable positive control you can test along side your trial antibody to make sure the technique works. Expensive, but I saved myself A HEAP of time that way.
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Old 03-18-2010, 06:54 AM
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Default Re: chIP antibodies

Hi, as you mentioned that you tried to get yourself a quick kit with a suitable positive control you can test along side your trial antibody to make sure the technique works.So,can I ask you how can I get a suitable positive control to check my technique?
Thank you~ ~
Ning
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Old 05-19-2010, 10:05 AM
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Default Re: chIP antibodies

Hi,
I'm having problems with my ab, I tried to add 5, 10, and didnt work but adding 20 mg worked. I'm just scared that is too much...
Any advice??
thank u very much
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