Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Epigenetics Forum: DNA Methylation, Histone and Chromatin Study > ChIP Chromatin Immunoprecipitation Forum
Register Search Today's Posts Mark Forums Read

ChIP Chromatin Immunoprecipitation Forum Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


need information

need information - ChIP Chromatin Immunoprecipitation Forum

need information - Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-08-2008, 07:47 PM
Pipette Filler
Points: 295, Level: 5 Points: 295, Level: 5 Points: 295, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2008
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default need information



I am planning to perform ChIP assay for the bacterial culture , kindly suggest me how many cells ...etc are to be taken ....i am pretty new to this assay.. going to perform for the first time.... hope to get your suggestions from you guys.

thanks
Reply With Quote
  #2  
Old 04-29-2009, 06:07 PM
Pipette Filler
Points: 74, Level: 1 Points: 74, Level: 1 Points: 74, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2009
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: need information

I was told 1x10^6 cells was appropriate for ChIP from mammalian cell lines if that's any help, but in truth I've successfully ChIP'ed with much less than this without refining the protocol.
I now ignore estimates like this altogether and work on the basis of the amount of chromatin I need. I add 50 micrograms of fixed, sheared chromatin to each of my ChIP reactions.
Reply With Quote
Reply

Tags
information


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Zbrafish Digest, Vol 28, Issue 3 raj abi Zebrafish Forum 0 09-07-2007 07:35 PM
Achtung! Troll im Tal (Die Information) Kasperl Obergscheit Forum Physik 16 10-30-2006 02:40 AM
Physics and Life - by Paul Davies Laurent Physics Forum 6 01-15-2004 04:17 PM
Sci.chem FAQ - Part 2 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:07 AM
Sci.chem FAQ - Part 1 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:06 AM


All times are GMT. The time now is 01:33 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13318 seconds with 16 queries