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| ChIP Chromatin Immunoprecipitation Forum Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more. |
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07-11-2008, 11:14 PM
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 ChIP Assay Sonication Problems Hello all, im getting problems with my ChiP sonication steps. I follow the steps from the Santa Cruz kit. Im using all the reagents according to the manufacturers instructions. The problem is that when I sonicate my 293T cell nuclear extracts, and then I reverse cross-link, and then phenol extract my DNA, I get only a DNA that smears. Can someone hand me some suggestions please? thanks        |
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