Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Chemistry Forum
Register Search Today's Posts Mark Forums Read

Chemistry Forum Chemistry Forum. Discuss chemical reactions, chemistry.


IEC-Removing NaOH

IEC-Removing NaOH - Chemistry Forum

IEC-Removing NaOH - Chemistry Forum. Discuss chemical reactions, chemistry.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-21-2013, 10:26 PM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2013
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default IEC-Removing NaOH



Hi all. I use 0.5 M NaOH for cleaning anion and cation exchange resins after protein purification and I need a quick/efficient way of removing the NaOH before storage in 20% ethanol. H2O does not work quickly enough - can take more than 10 column volumes. I think I need a buffer at high concentration (1M) that can displace the OH- ions quickly, then I would follow with H2O (so salts won't precipitate when ethanol is used later for storage). I'm hoping to use maybe 1 column volume of high concentration buffer and 2-3 column volumes of H2O. Which buffer(s) would work well? I would prefer to use the same buffer for all cation and anion resins if possible for convenience. Any advice?
Reply With Quote
Reply

Tags
iecremoving , naoh


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Steżenie NaOH adaś Forum Chemia 0 09-01-2005 09:02 PM
Steżenie NaOH adaś Forum Chemia 0 09-01-2005 08:59 PM
Jaka szkodliwoœć FeCl3 , NaOH szlovak Forum Chemia 13 04-21-2005 10:02 PM
Aluminium polieren mit NaOH Andreas Pietruschka Forum Chemie 10 08-20-2004 11:56 AM
Bezugsquellen für NaOH in Lebenbsmittelqual. Tobias Grassl Forum Chemie 54 08-20-2003 12:28 PM


All times are GMT. The time now is 08:18 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11216 seconds with 16 queries