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PJB245 01-21-2013 02:57 AM

Maldi
 
When I run my MALDI analysis, I only detect matrix peaks in my samples. My samples are short homooligomeric peptides of random lengths (n= 5 to 15) composed of hydrophobic amino acids. To solublize my peptides, I use 0.5% formic acid and sonicate them at 40oC. I have tried Zip Tip and the matrix I have been using is alpha-CHCA. Any advice would be great.


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