glycine neutralize formaldehyde

[memari]

In Biology there is a protocol with the name of CHIP.
In this protocol, biologists use glycine to neutralize formaldehyde.
I have read a lot of chip protocols and all of them use 125mM Glycine.

Why should I use glycine to a final concentration of 125mM ? why not more or less?
I wonder why all use this amount of glycine. why not 200mM, or 500mM?
Is it just an Imitation?or is there any chemical reason behind it?

-----
Babak

[mmorgan]

Originally Posted by memari

In Biology there is a protocol with the name of CHIP.
In this protocol, biologists use glycine to neutralize formaldehyde.
I have read a lot of chip protocols and all of them use 125mM Glycine.

Why should I use glycine to a final concentration of 125mM ? why not more or less?
I wonder why all use this amount of glycine. why not 200mM, or 500mM?
Is it just an Imitation?or is there any chemical reason behind it?

-----
Babak

Glycine quenches the formaldehyde

[memari]

I am sure that using 125mM is just an imitation with blind eye.
One molecule of Formaldehyde quenches one molecule of Glycine. so when we use 125mM glycine, it is good just for 125mM of Formaldehyde. 125mM formaldehyde is 0.375%.

[Only registered users see links. ]

One 1% of Formaldehyde is 0.33M.

But biologists always use 125mM glycine for all different concentration of Formaldehyde.
Do not you think that they (biologists ) are wrong?

125mM glycine is good just for 0.375 % of formaldehyde.

What is your idea?

-----
Babak