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09-28-2011, 03:06 AM
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 Analysis of cyclobutane pyrimidine dimers Hi~~ I have some nucleic samples and I believe cyclobutane pyrimidine dimers are present after a certain type treatment. Does anyone know of a method to see the formation of such bondings at an atomic level? Or is there a specific way to check the presence of cyclobutane pyrimidine dimers? Note: People have been using HPLC-MS/MS for such an application but no protocol has been developed for the type of sample I am using. I might be able to adopt the protocol of a similar type of sample but I doubt the results. Other possible methods are circular diachroism, NMR, SAXS. All these methods are based on the light absorption or neutron/X-ray reflection/diffraction pattern of the sample, which, to me, is only an indirect readout of the structure of the molecule.  |
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| analysis , cyclobutane , dimers , pyrimidine |
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