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Serial Dilution Dilemma

Serial Dilution Dilemma - Chemistry Forum

Serial Dilution Dilemma - Chemistry Forum. Discuss chemical reactions, chemistry.


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  #1  
Old 01-15-2011, 10:25 PM
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Default Serial Dilution Dilemma



Hello, I am undergraduate student and need a bit of help.

Specifically, I would appreciate your assistance in helping me confirm how to do serial dilutions to obtain 2 specific concentrations.

Resveratrol has a molar mass of 228 g/mol.
it can be dissolved in 16 mg/ml DMSO. This is the minimal volume for it to be soluable.

if we cross multiply we can determine that the stock concentration is 70 mM. This solution will be my 'master mix'. For the first run i only need to apply 10mM and 40 mM of variable concentration of resveratrol to my cells.

So....how would I do this? I dont think that this is particularly difficut, but yet I am struggling a bit.

Please let me know how to do this and comment on the initial mass and volumes I should use. Thanks!
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  #2  
Old 01-20-2011, 02:55 AM
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Default Re: Serial Dilution Dilemma

Search the forum for the thread: "How to prepare a buffer". There I describe a useful formula which will help you with this and many other similar dilutions.
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Old 01-20-2011, 06:14 AM
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Default Re: Serial Dilution Dilemma

Hola, Luisillo shows to you how to fish, and I will give you the fishes, that is other way to learn fishing. Well, you have 70mM solution; if you mix 600ul of this with 100ul of buffer ,media or DMSO you will have a 60mM solution. 500+200=> 50mM, 400+300=>40mM, etc untill 100+600=> 10mM. If you need less volume 60+10=> 60mM ..........10+60=> 10mM. Good luck
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Old 01-20-2011, 07:24 PM
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Default Re: Serial Dilution Dilemma

Supongo que la mayoría de las personas busca que "le den pescado" cuando postea en el foro. Yo también suelo hacerlo, aunque en ésta ocasión me encontraba en un cyber-café donde no me sentía muy concentrado.

De cualquier modo, protolder te agradezco por haber tenido la molestia de darle el lugar a mi sugerencia.

Saludos

Last edited by luisillo; 01-20-2011 at 07:25 PM. Reason: Some spelling mistakes
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dilemma , dilution , dmso , factor , resveratrol , serial


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