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How to make stock solution?

How to make stock solution? - Chemistry Forum

How to make stock solution? - Chemistry Forum. Discuss chemical reactions, chemistry.


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  #1  
Old 03-02-2010, 04:04 AM
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Default How to make stock solution?



Hi, I need to dissolve curcumin in 0.5% DMSO. Does that means 100mg of curcumin in 0.5mL DMSO?
Thanks
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Old 03-02-2010, 04:22 AM
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Default Re: How to make stock solution?

No you can make 0.5% DMSO by diluting into water.
Their densities are about the same so 0.5% v/v or w/w will be very similar anyway.

You should figure out the concentration of curcumin before deciding the volume of 0.5% DMSO.
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Old 03-02-2010, 06:33 AM
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Default Re: How to make stock solution?

I usually dissolve 0.02g extract in 1000 microlit pure DMSO as stock solution.

For dilution, I add 10 microlit of the stock to 90 microlit of 10% DMSO in tubes. From there, I transfer 10 microlit from the tube + 190microlit cell suspension in the well. This will gives me 100mcg/mL concentration.

But I have read in the forum shouldn't exceed 0.5% DMSO for cell culture work. My problem is how do I calculate the final concentration of DMSO here?

Sorry. Sounds very stupid but my chemistry is very rusty.
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Old 03-02-2010, 04:38 PM
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Wink Re: How to make stock solution?

Quote:
Originally Posted by mcdeena View Post
I usually dissolve 0.02g extract in 1000 microlit pure DMSO as stock solution.

For dilution, I add 10 microlit of the stock to 90 microlit of 10% DMSO in tubes. From there, I transfer 10 microlit from the tube + 190microlit cell suspension in the well. This will gives me 100mcg/mL concentration.

But I have read in the forum shouldn't exceed 0.5% DMSO for cell culture work. My problem is how do I calculate the final concentration of DMSO here?

Sorry. Sounds very stupid but my chemistry is very rusty.
I'll try to calculate, for you

stock = 1 ml 100% DMSO (w/curcumin dissolved)
1st dilution 1:10 = 10% DMSO
2nd dilution (into cell culture) 1:20 = 10% *1/20 = 0.5% DMSO (w/curcumin dissolved)

for curcumin conc'n in g
stock = 0.02g/1ml
1st dil'n 1:10 = 0.002g/ml
2nd diln 1:20 = 0.002g/ml *1/20= 0.0001g/ml or 0.1 mg/ml

Hope that helps.
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Old 03-02-2010, 04:46 PM
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Cool Re: How to make stock solution?

Quote:
Originally Posted by mcdeena View Post

For dilution, I add 10 microlit of the stock to 90 microlit of 10% DMSO in tubes. From there, I transfer 10 microlit from the tube + 190microlit cell suspension in the well. This will gives me 100mcg/mL concentration.
Didn't notice that underlined part the first time.
I don't think you need to dilute into 10% DMSO just use molecular grade water.
I think you are ending up with

%DMSO = (10 *100%/100) + (90*10/100) = 10% + 9% = 19%DMSO at 1st dilution.
Makes sense because 90ul at 10% would be 9ul of DMSO + 10ul 100% DMSO = 19ul of DMSO / 100ul total volume

Like I said you don't need to dilute into 10% solution, unless you can also dissolve the curcumin into pure water, then your method would work and give you the 0.5% DMSO final conc'n.
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Old 03-05-2010, 03:19 AM
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Default Re: How to make stock solution?

Danfive, thanks so much for your reply and the info. Yup, I managed to calculate the concentration of DMSO and figured out it's 0.5% as well Cheers.
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Old 04-09-2010, 05:40 AM
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Default Re: How to make stock solution?

same question to you,but i think we can resolve it in this problem
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Old 04-15-2010, 12:41 PM
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Default Re: How to make stock solution?

Dissolve 0.3 grams in 1 L.
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Old 04-15-2010, 03:54 PM
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Thumbs down Re: How to make stock solution?

Quote:
Originally Posted by AllyAshantiBrown View Post
Dissolve 0.3 grams in 1 L.
WTF?
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Old 07-20-2011, 11:01 AM
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Default Re: How to make stock solution?

Hi, I would like to know the best way to treat my cancer cells for apoptosis (in tissue culture dishes with 10^6 cells) with IC50 or should I use various concentrations? I have tried to treat with my IC50 and run the cell cycle analysis (24hrs), there is no cell death at G0.
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