10 mM HCl for IGF-I

Hi,

I need some 10 mM HCl to reconstitute IGF-I and was wondering if it is
something that can be purchased or does it have to be made, and what
procedure and other compounds I would need in order to make?

BTW - Here is a list of the different types of HCl available to me, is it
possible that any of them could be used in place of 10 mM HCl

Hydrochloric Acid
Hydrochloric Acid Concentrate
Hydrochloric Acid Concentrate, 1N
Hydrochloric Acid R1 (250 g/L HCl)
Hydrochloric Acid Solution
Hydrochloric Acid, 0.0100 Normal (N/100)
Hydrochloric Acid, 0.0200 Normal (N/50)
Hydrochloric Acid, 0.0500 Normal (N/20)
Hydrochloric Acid, 0.05882 Normal (N/17)
Hydrochloric Acid, 0.100 Normal (N/10)
Hydrochloric Acid, 0.100 Normal (N/10) in Isopropyl Alcohol
Hydrochloric Acid, 0.1142 Normal, for Kjeldahl Analysis of Proteins
Hydrochloric Acid, 0.1782 Normal, 1 mL = 5 mg CaO (Lime)
Hydrochloric Acid, 0.200 Normal (N/5)
Hydrochloric Acid, 0.200 Normal (N/5) in Isopropyl Alcohol
Hydrochloric Acid, 0.250 Normal (N/4)
Hydrochloric Acid, 0.250 Normal (N/4) in Isopropyl Alcohol
Hydrochloric Acid, 0.300 Normal
Hydrochloric Acid, 0.500 Normal (N/2)
Hydrochloric Acid, 0.500 Normal (N/2) in Isopropyl Alcohol
Hydrochloric Acid, 0.600 Normal
Hydrochloric Acid, 1% (v/v) Aqueous Solution (1 + 99)
Hydrochloric Acid, 1.00 Normal
Hydrochloric Acid, 1.5% (v/v) Aqueous Solution
Hydrochloric Acid, 1.96% (v/v) Aqueous Solution (1 + 50)
Hydrochloric Acid, 10% (v/v) Aqueous Solution (1 + 9)
Hydrochloric Acid, 10.0 Normal
Hydrochloric Acid, 2% (v/v) Aqueous Solution (1 + 49)
Hydrochloric Acid, 2.00 Normal
Hydrochloric Acid, 2.50 Normal
Hydrochloric Acid, 20% (v/v) Aqueous Solution (1 + 4)
Hydrochloric Acid, 25% (v/v) Aqueous Solution (1 + 3)
Hydrochloric Acid, 25% v/v (1+3)
Hydrochloric Acid, 25% v/v (1+3)
Hydrochloric Acid, 3.00 Normal
Hydrochloric Acid, 5% (v/v) Aqueous Solution (1 + 19)
Hydrochloric Acid, 5.00 Normal
Hydrochloric Acid, 50% (v/v) Aqueous Solution (1 + 1)
Hydrochloric Acid, 6.00 Normal
Hydrochloric Acid, 6.00 Normal, Packaged in Glass
Hydrochloric Acid, 6.25 Normal
Hydrochloric Acid, 75% (v/v) Aqueous Solution (3 + 1)
Hydrochloric Acid, 8.33% (v/v) Aqueous Solution (1 + 11)
Hydrochloric Acid, Dilute R (73 g/L HCl)

Thank you,
David Low

"low.d" <[Only registered users see links. ]> wrote in message news:<Eshdb.444415$cF.142070@rwcrnsc53>...
(snip)


Yes, any of these could be diluted to 10 mM. But why would you want
to formulate your own drugs, when safely formulated products are
already on the market? And why would you want to take this one in
particular, which is the subject of so many bogus marketing claims?

[Only registered users see links. ]

Furthermore, HCl is a rather dangerous acid. Imagine if you do not
dilute it to the correct concentration, then injest it, inhale it, or
inject it. You might die. Someone you know might die. Or you might
go to prison. Don't do it.

Dave

"dave e" <[Only registered users see links. ]> wrote in message
news:10ffa4e4.0309271425.293cbc63@posting.google.c om...

Dave, I appreciate your concern. What safely formulated drugs are you aware
of that can mimic the effects of IGF-I? The article you referenced relates
to r-hGH and not IGF-I.

If I was to use HCl acid, I may well injure myself if I am stupid, but I
think that death is not likely. If 10 mM HCl does not harm cell cultures,
why would it injure me? I am confused how my actions could kill someone I
know or land me in jail? I am not working with a scheduled or listed
substance, nor am I planning to do anything illegal.

Thanks,
David

"low.d" <[Only registered users see links. ]> wrote in message news:<2Xqdb.168797$[Only registered users see links. ].asp.att. net>...

IGF-1 itself is available as a dietary supplement, from many of the
same supplement companies which sell HGH supplements. And while I am
skeptical of the supplement industry in general (since the industry is
almost completely unregulated) they do, sometimes, hire experienced
chemists to safely formulate their products.

My concern, when I read your message, is that you don't sound like an
experienced chemist. Therefore, it would be particularly unsafe for
you to mix chemicals which you plan to later put in your body, or sell
to another person to put in theirs.

The article I posted is indeed about HGH, rather than IGF 1
specifically, however the two hormones are physiologically related.
HGH exerts its effect by stimulating the production of IGF 1 in the
liver. Several of the bogus claims which were earlier being made
regarding HGH are now being made regarding IGF 1. They are still
bogus. IGF 1 is a polypeptide, just like HGH, and neither of these
will enter the blood stream if taken orally.

If you are planning to take the stuff intravenously, then obviously,
you must be even more cautious about mixing your own.

My comment about the possibility that you might "go to jail" was
related to an assumption I made that you planned to sell this stuff to
others. If you were to sell it to another, and caused them injury,
you could be held liable.

Dave

"Bob" <[Only registered users see links. ]> wrote in message
news:[Only registered users see links. ]...
cultures,

Bob, are you sure? The company that patented and manufactures the specific
IGF-I that I am interested in, specifically to promote growth in cell
cultures, recommends that you reconstitute it with 10mM HCl before adding it
to your cell cultures? As do their exclusive distribution partner in the
USA.

David.

"dave e" <[Only registered users see links. ]> wrote in message
news:10ffa4e4.0309280407.238c799a@posting.google.c om...

Dave,

I certainly do not plan to sell anything, nor would I ever inject it
intravenously.

You are correct, I am not an experienced chemist, which is why I came here
for some assistance. I am aware of the supplements that claim to increase
hGH or IGF-I, but I also know them to be bogus, I though you were aware of a
drug that would do so, perhaps one which I was not familiar with.

Right now IGF-I is available on the 'black-market, in fact it has been for
several years. Previously, it was regular IGF-I and came with a vial of
10mM HCl or 100mM acetic acid for reconstitution, presently all that is
readily available is Long R3 IGF-I which comes already reconstituted in
benzyl alcohol. Ignoring the debate on safety, potential side effects or
whether it actually works or not, I do not believe that you can suspend a
polypeptide like IGF-I in benzyl alcohol with degrading or killing it. I
hope this explains my interest.

Thanks,
David.

"Bruce Hamilton" <[Only registered users see links. ].nz> wrote in message
news:[Only registered users see links. ]...
water.

Thank you.

"low.d" <[Only registered users see links. ]> schrieb im Newsbeitrag
news:Eshdb.444415$cF.142070@rwcrnsc53...
10mM means 0.3646g (10mMole) of HCl (M= 36.46 g/Mol) dissolved in 1L of
water.
Concentrated HCl usually comes as 10M solution.


What do you need this IGF-I (whatever it is good for?) anyways?

MfG
Chris

In article <4aBdb.171752$mp.92907@rwcrnsc51.ops.asp.att.net >, "david" <david@comcast.net> wrote:

Depends quite a lot on how much you add, and how buffered the cell culture
medium is. IIRC the 10 mmoles/L has been the standard prep method for a long
time. Consider ... if you are then diluting it (as is likely) before use ...
and then adding small amounts (5-10 uL/well ?)

Shouldn't be a problem ... but testing is your friend

Bruce




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in article [Only registered users see links. ], dave e at
[Only registered users see links. ] wrote on 9/27/03 3:25 PM:


It is not as dangerous as you make out. The hydrochloric acid in your
stomach is probaby stronger than (I did not bo9ther to look it up) than 10
mM. Impurities may be a bigger problem than the acid. Fully ionized and
active, 10 mM should give a pH of 2.

Bill