Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Chemistry Forum
Register Search Today's Posts Mark Forums Read

Chemistry Forum Chemistry Forum. Discuss chemical reactions, chemistry.

Fatty acids: analysis techniques?

Fatty acids: analysis techniques? - Chemistry Forum

Fatty acids: analysis techniques? - Chemistry Forum. Discuss chemical reactions, chemistry.

LinkBack Thread Tools Display Modes
Old 07-17-2003, 06:19 PM
Posts: n/a
Default Fatty acids: analysis techniques?

[Only registered users see links. ] (MC) wrote in message news:<[Only registered users see links. ].uk>...

GC/MS based on EPA method 8270 (Semi-volatiles) should work fine. You
would, of course, need to verify the ID's and do proper validation,
but, on the face of it the method should handle what your looking for.
There are HPLC methods as well, but I prefer GC/MS since the
technology is so pervasive.

Method can be found at
[Only registered users see links. ].

Good Luck.

Reply With Quote
Old 07-18-2003, 01:41 PM
Dr. George O. Bizzigotti
Posts: n/a
Default Fatty acids: analysis techniques?

On 17 Jul 2003 11:19:33 -0700, [Only registered users see links. ] (bwh) wrote:

It depends on what you mean by "based on." Method 8270 would almost
certainly detect the fatty acids as tentatively identified compounds
based on MS library matching; it depends on having spectra in the
library, which can vary to some degree. However, the quantities will
be qualified as estimated, because 8270 does not incluse fatty acids
in the calibration standards. One could modify the calibration
standards, but then one is into method development, which the original
poster is presumably looking to avoid.

One source that I have not seen anyone mention yet is the Official
Methods and Recommended Practices of the AOCS, 5th edition, American
Oil Chemists Society), e.g., those for derivitization as the methyl
ester (AOCS Ce 2-66) folowed by determination by gas chromatography
(AOCS Ce 1-62). They have a web site at
[Only registered users see links. ] that allows one to
search their methods to firnd the definition and scope. They also
deliver the full methods electronically for a fee.


************************************************** ********************
Dr. George O. Bizzigotti Telephone: (703) 610-2115
Mitretek Systems, Inc. Fax: (703) 610-1558
3150 Fairview Park Drive South E-Mail: [Only registered users see links. ]
Falls Church, Virginia, 22042-4519
************************************************** ********************

-----= Posted via Newsfeeds.Com, Uncensored Usenet News =-----
[Only registered users see links. ] - The #1 Newsgroup Service in the World!
-----== Over 80,000 Newsgroups - 16 Different Servers! =-----
Reply With Quote

acids , analysis , fatty , techniques

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Fatty acids: analysis techniques? Mark Tarka Chemistry Forum 2 09-12-2013 01:21 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Sci.chem FAQ - Part 7 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:13 AM
Fatty acids: analysis techniques? Bruce Hamilton Chemistry Forum 2 07-20-2003 12:17 PM
Fatty acids: analysis techniques? Andrew P Chemistry Forum 0 07-17-2003 06:08 PM

All times are GMT. The time now is 04:01 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14655 seconds with 16 queries