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Cytokines feeding in cell culture

Cytokines feeding in cell culture - Cell Biology and Cell Culture

Cytokines feeding in cell culture - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 09-14-2013, 08:20 PM
Pipette Filler
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Default Cytokines feeding in cell culture



Hi everybody,

I need to add cytokines to my cell cultures and in some protocols say I should add certain amount of cytokines per number of cells and in other protocols only refer to cytokines concentration in the culture.

If I consider cytokine concentration it looks it is irrelevant the cell number I have in the culture. But maybe for stimulation with cytokines it is in fact important the amount of cytokines per number of cells.

I would appreciate your comments! thanks!
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Old 02-25-2014, 09:49 AM
Pipette Filler
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Default Re: Cytokines feeding in cell culture

I would use a set concentration of cytokines, rather than basing it on a number of cells. If these are cell cultures then they are probably going to be growing and dividing, so the number at plating will not be the number later in your experiment.
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