Cytokines feeding in cell culture
I need to add cytokines to my cell cultures and in some protocols say I should add certain amount of cytokines per number of cells and in other protocols only refer to cytokines concentration in the culture.
If I consider cytokine concentration it looks it is irrelevant the cell number I have in the culture. But maybe for stimulation with cytokines it is in fact important the amount of cytokines per number of cells.
I would appreciate your comments! thanks!
Re: Cytokines feeding in cell culture
I would use a set concentration of cytokines, rather than basing it on a number of cells. If these are cell cultures then they are probably going to be growing and dividing, so the number at plating will not be the number later in your experiment.
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