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-   -   Histone H1 isolation - avoiding acidic conditions (http://www.molecularstation.com/forum/cell-biology-cell-culture/87905-histone-h1-isolation-avoiding-acidic-conditions.html)

Valine 06-02-2013 07:12 PM

Histone H1 isolation - avoiding acidic conditions
 
Dear all,

First of all I have to say that I am not really experienced with cells and proteins isolation.

My question is that I wanna isolated Histone H1 from a type of cells. There are a lot of reports, but all of them used acidic conditions (perchlorid acid, sulfuric acid, trichloroacetic acid...) and thats something that I would like to avoid. In some of the cases I understand that such conditions are used to precipitate the proteins and in others I guess that are to break/lyse the cell membrane nucleus.

Are u aware of any method to isolate proteins from the nucleus avoiding acidic conditions?

And second question, why such acids are being used to isolated such proteins (in case that my previous afirmations are wrong)?

Thanks very much!


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