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Cell staining for Fluorometric Assay
I'm trying to develop a fluorometric assay to monitor the expression of a surface protein in HL-60 cells, using fluorescence antibody. We would like to use a fluorescence plate reader to measure the fluorescence of antibodies bound to cells. Could you recommend me some protocol to stain cells plated in a 96-well microplate?Do you have a recipe for PBS buffer supplemented with FBS and NaN3?
Any suggestions would be greatly appreciated!
|assay , cell , fluorometric , staining|
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