Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture
Register Search Today's Posts Mark Forums Read

Cell Biology and Cell Culture Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


cell counting

cell counting - Cell Biology and Cell Culture

cell counting - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-24-2012, 02:01 PM
Pipette Filler
Points: 68, Level: 1 Points: 68, Level: 1 Points: 68, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2012
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default cell counting



Hi everybody, I need help in my cell counting. I'm trying to count cells (Neubauer chamber) using Trypan Blue. I use 1 microliter of cells suspension and 9 microliters of Trypan Blue. Do somebody of you tell me how count in this chamber?
I counted in a wrong manner for one year!!!
Please help me!
Reply With Quote
  #2  
Old 05-24-2012, 02:16 PM
Pipette Filler
Points: 68, Level: 1 Points: 68, Level: 1 Points: 68, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2012
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: cell counting

Sorry another question: how you calculate time of duplication of your cell population? I use n= (log N2- log N1)/log2
g=t/n

where N2= cells number at finish
N1= cells number at start
g= time of generation
t= time

but, I think that there is need of a coefficient for area change. Am I in error?
Reply With Quote
Reply

Tags
cell , counting


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
A Human Cytome Project - an idea - Update 14 March 2005 Peter Van Osta Cell Biology and Cell Culture 0 03-14-2005 02:27 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
Tissue Engineering & Stem Cell Research 2005 Andrew Oliver Protocols and Methods Forum 0 12-02-2004 11:38 PM


All times are GMT. The time now is 12:52 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16608 seconds with 16 queries