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#1
05-24-2012, 02:01 PM
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 cell counting Hi everybody, I need help in my cell counting. I'm trying to count cells (Neubauer chamber) using Trypan Blue. I use 1 microliter of cells suspension and 9 microliters of Trypan Blue. Do somebody of you tell me how count in this chamber? I counted in a wrong manner for one year  !!!Please help me!  |
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#2
05-24-2012, 02:16 PM
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| Re: cell counting Sorry another question: how you calculate time of duplication of your cell population? I use n= (log N2- log N1)/log2 g=t/n where N2= cells number at finish N1= cells number at start g= time of generation t= time but, I think that there is need of a coefficient for area change. Am I in error? |
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