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Heat lysis of mammalian cells

Heat lysis of mammalian cells - Cell Biology and Cell Culture

Heat lysis of mammalian cells - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 03-01-2012, 09:12 PM
Pipette Filler
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Default Heat lysis of mammalian cells



Hello,


I could use some advice: do you think that heating standard mammalian cells that have been highly diluted to 65 C for 10 min would lyse their membranes? Seems like it should but I'm confused why there are all these kits out there for lysis of a small number of cells when it seems like a small amount of heat should do the trick.


Thanks!
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