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CHO cells aren't flat enough, won't stick well.

CHO cells aren't flat enough, won't stick well. - Cell Biology and Cell Culture

CHO cells aren't flat enough, won't stick well. - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 10-06-2011, 05:24 PM
Pipette Filler
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Default CHO cells aren't flat enough, won't stick well.



I am trying to grow CHO cells for nuclear targeted beta-galactosidase and immunohistochemistry studies. However, I am having trouble getting the cells to grow flat, they ball up and distinguishing the nucleus from the rest of the cell is difficult. They grow well on 6 well plates treated with vacuum gas plasma, but the plates don't work well on microscopes. I have tried to grow the CHO cells on BD poly-lysine cover slips in the 6 well plates, but they seem to ball up on that too. I have not tried a collagen coated cover slip yet, but am thinking about it. The information with the poly-lysine cover slips doesn't say if they're coated on one side or both sides. I suppose I could be placing them all upside down, but I can't tell which side is which if it matters.

Has anyone here grown CHO cells flat for microscopy uses?

Also, is there a better way to pull the cover slips back out of the wells after the cells grow? Even with forceps catching the cover slips is hard.
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Old 10-19-2011, 04:32 PM
Pipette Filler
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Default Re: CHO cells aren't flat enough, won't stick well.

Just an update, I also tried collagen coated coverslips and the cells are still round. I don't know how to fix this. May have to acid etch some coverslips and see if that helps.

Also trying to grow HepG2 cells, and they're growing clumpy. I split them every third day and try to trypsanize them fairly heavily to break up the clumps. I want them to grow in a flat layer so I can see them well in the same nuclear targeting assay. Any hints on getting them to grow flat?
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