I am trying to grow CHO cells for nuclear targeted beta-galactosidase and immunohistochemistry studies. However, I am having trouble getting the cells to grow flat, they ball up and distinguishing the nucleus from the rest of the cell is difficult. They grow well on 6 well plates treated with vacuum gas plasma, but the plates don't work well on microscopes. I have tried to grow the CHO cells on BD poly-lysine cover slips in the 6 well plates, but they seem to ball up on that too. I have not tried a collagen coated cover slip yet, but am thinking about it. The information with the poly-lysine cover slips doesn't say if they're coated on one side or both sides. I suppose I could be placing them all upside down, but I can't tell which side is which if it matters.
Has anyone here grown CHO cells flat for microscopy uses?
Also, is there a better way to pull the cover slips back out of the wells after the cells grow? Even with forceps catching the cover slips is hard.