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Intact Mitochondria isolation

Intact Mitochondria isolation - Cell Biology and Cell Culture

Intact Mitochondria isolation - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #21  
Old 10-19-2011, 06:31 AM
Pipette Filler
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Default Re: Intact Mitochondria isolation

I found a protocol which is adapted from Attardi and Ching (1979) and Rice and Lindsay (1997), is designed for cultured cells (2.10^8) grown as a monolayer. It's really close to your protocol. I hope it will be enough pure for my EM experiement
thanks
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  #22  
Old 10-20-2011, 08:28 AM
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Default Re: Intact Mitochondria isolation

Ok i have succeed to obtain mitochondria, apparently my problem was the concentration of cells, I increase by 3 the number and that's work with differential centrifugation. Thank you very much for your help. I have a last question do you know what kind of compound can I use to replace the sugar in the buffer ( sucrose and manitol) and keep the same osmolarity.

thanks

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  #23  
Old 12-20-2011, 10:03 AM
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Default Re: Intact Mitochondria isolation

Thank you very much for your help. I have a last question do you know what kind of compound can I use to replace the sugar in the buffer ( sucrose and manitol) and keep the same osmolarity and how can I prevent the agregation of mitochondria.

thanks
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  #24  
Old 12-20-2011, 03:53 PM
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Default Re: Intact Mitochondria isolation

You may be able to do it with a protein like BSA; however, you'll need a osmometer to accurately measure the osmolarity.

Bryan
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  #25  
Old 01-09-2012, 09:45 AM
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Default Re: Intact Mitochondria isolation

Thank you very much I will try immediatly
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  #26  
Old 02-22-2012, 07:45 PM
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Default Re: Intact Mitochondria isolation

thanks
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  #27  
Old 08-29-2012, 11:13 PM
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Default Re: Intact Mitochondria isolation

Hello everybody!Does anybody know which cell line can I use for import of radiolabeled precursors in human for example mitochondria?They must be pure, must not they?
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  #28  
Old 02-11-2013, 12:35 PM
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  #29  
Old 02-11-2013, 12:43 PM
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