I have been trying to grow HCC827GR6 cells. These cells appear to be extremely sensitive. I was asked to not let them trypsize too long. Hence i did that but my concenrn is that if I dont let them trypsinize long enough, not all cells will come off the base.
Also, these cells tend to clump up upon trypsinization. I dont trypsinize them any more than 5 min. I dont want to piptte them too many times to dislodge them either.
Has any one worked with them and had any issues like these?
And what would be the best way to treat this sensitive cell line?
Thank you so much