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help: EMT and MDCK cells

help: EMT and MDCK cells - Cell Biology and Cell Culture

help: EMT and MDCK cells - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 05-28-2011, 10:06 AM
Pipette Filler
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Smile help: EMT and MDCK cells



Hello to everybody,

Is there somebody studying Epithelial to Mesenchymal transition?

My question is about TGFb1-dependent EMT induction in MDCK cells:

1) do they have to be at high confluence when stimulated?
2) do you keep serum during stimulation?
3) after how many days you get a morphologycal phenotype?

Thanks for help,

Marco
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Old 12-08-2011, 02:20 AM
Pipette Filler
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Default Re: help: EMT and MDCK cells

I would like to know the answer to this too!
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