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help: EMT and MDCK cells
Hello to everybody,
Is there somebody studying Epithelial to Mesenchymal transition?
My question is about TGFb1-dependent EMT induction in MDCK cells:
1) do they have to be at high confluence when stimulated?
2) do you keep serum during stimulation?
3) after how many days you get a morphologycal phenotype?
Thanks for help,
|cells , emt , mdck|