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Harvesting cells for DNA/RNA extraction. HOW?

Harvesting cells for DNA/RNA extraction. HOW? - Cell Biology and Cell Culture

Harvesting cells for DNA/RNA extraction. HOW? - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 05-13-2011, 01:48 PM
Pipette Filler
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Default Harvesting cells for DNA/RNA extraction. HOW?



Hi,

I have cell lines I treated with a reagent and want to harvest them now in order to extract DNA and RNA. My problem now: can I scrap them or do I use trypsine? If I use trypsine does it affect the DNA extraction later (Phenol-Chloroform Method). Actually I did the harvesting with PBS and a cell scraper but it was for Western Blots.

And which speed can I use for the centrifugation step?

Thanks a lot in advance I need to harvest my cells in 24 hrs so PLSSSSs help me !!!!
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Old 08-03-2011, 05:47 PM
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Default Re: Harvesting cells for DNA/RNA extraction. HOW?

Nice!
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Old 08-05-2011, 02:07 AM
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Default Re: Harvesting cells for DNA/RNA extraction. HOW?

Go ahead. Nucleic acid extraction requires cell lysing, so I don't think scrapping the cells could detrimental to the experiment. I don't know about trypsine in DNA extraction, but I'd go for the scrapping, although an EDTA solution might come in handy while scrapping the cells in order to keep nuclease activity at minimum. Being quick also helps.

Good luck.
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