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Immunoglobulins in cell culture.

Immunoglobulins in cell culture. - Cell Biology and Cell Culture

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  #1  
Old 04-28-2011, 04:38 PM
Pipette Filler
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Default Immunoglobulins in cell culture.



How long does it take for lymphocyte B to produce antibodies in vitro? I lead 5-day-long culture stimulated with anti-CD3. B-cells proliferate about up to 7 times after 5 days in my culture and later I want to check the level of antibodies by ELISA (I collect supernatants from certain days of culture). I am wondering if it is not too short culture. I read articles about anitbodies production and it seems that for each factor (antigen) the production of antibodies takes different time. I stimulate B-cells through stimulation of T-cells by anti-CD3 so it is nothing new for the cells. Should be quite fast production of antibodies, but I am not sure if in my cell culture I am able to catch any antibodies. Anybody?

Last edited by konfolut; 04-28-2011 at 04:40 PM. Reason: mixed days of culture
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Old 04-28-2011, 08:43 PM
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Default Re: Immunoglobulins in cell culture.

Considering the literature I've read, 3-5 days are ok for IgM production; and for IgG production I've read you need 7-10 days of culture. I don't know for the other isotypes.
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Old 04-29-2011, 01:04 PM
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Default Re: Immunoglobulins in cell culture.

Quote:
Originally Posted by luisillo View Post
Considering the literature I've read, 3-5 days are ok for IgM production; and for IgG production I've read you need 7-10 days of culture. I don't know for the other isotypes.
Can you give me links to the literature you read?
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Old 05-01-2011, 06:43 PM
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Default Re: Immunoglobulins in cell culture.

It's a bit old though:

[Only registered users see links. ]

I got some of the info from "Current protocols in immunology", can't remember the tome, though.
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Old 08-03-2011, 05:46 PM
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Default Re: Immunoglobulins in cell culture.

thanks!
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antibody production , cell , cell culture , culture , duration , immunoglobulins , time


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