Immunoglobulins in cell culture.

[konfolut]

How long does it take for lymphocyte B to produce antibodies in vitro? I lead 5-day-long culture stimulated with anti-CD3. B-cells proliferate about up to 7 times after 5 days in my culture and later I want to check the level of antibodies by ELISA (I collect supernatants from certain days of culture). I am wondering if it is not too short culture. I read articles about anitbodies production and it seems that for each factor (antigen) the production of antibodies takes different time. I stimulate B-cells through stimulation of T-cells by anti-CD3 so it is nothing new for the cells. Should be quite fast production of antibodies, but I am not sure if in my cell culture I am able to catch any antibodies. Anybody?

[luisillo]

Considering the literature I've read, 3-5 days are ok for IgM production; and for IgG production I've read you need 7-10 days of culture. I don't know for the other isotypes.

[konfolut]

Originally Posted by luisillo

Considering the literature I've read, 3-5 days are ok for IgM production; and for IgG production I've read you need 7-10 days of culture. I don't know for the other isotypes.

Can you give me links to the literature you read?

[luisillo]

It's a bit old though:

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I got some of the info from "Current protocols in immunology", can't remember the tome, though.

[bushidodadon475]

thanks!