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TheNakedCell 04-13-2011 07:04 AM

synthetic alternatives for FBS
 
Hi all, we are new to this forum and are introducing a synthetic replacement for FBS; we would like to learn what you would like for such a product. Looking forward to your replies!

protolder 04-13-2011 07:55 AM

Re: synthetic alternatives for FBS
 
Hola, Ill give you my opinion, animal sera complements the formulation of a medium with growth factors, lipids, hormones, and other requierements, for growth. Serum replacements are used in well defined strategies as protein secretion were you need a cleaner extracellular medium, but it has an step of adaptation and the cost of subtitution could increase against a normal serun,moreover now , when a high % of expressed proteins have an specific tag to drive purification. There are some serum replaceemnts in the market as some cellular lines adapted to serum-free medium or protein free media. So your sinthetic serum could be of application depending of the final cost comparing with commmercial animal sera,and the levels of cellular density obtained. Buena suerte for this challenge.

TheNakedCell 04-13-2011 08:26 AM

Re: synthetic alternatives for FBS
 
Hi Protolder,

tx for your remarks, we are looking at a one-on-one replacement to FBS with no/little adaption and I will pass these comments to our chief scientist. Best regards/Diederik

bushidodadon475 08-03-2011 05:44 PM

Re: synthetic alternatives for FBS
 
nice!

valerianisman 10-24-2012 04:03 PM

Re: synthetic alternatives for FBS
 
Hi. I would be interested in learning more about this product. I am looking for a good synthetic (and cruelty-free) sustitute for FBS. Thank you.

jcoalsonshore 11-08-2012 02:49 PM

Re: synthetic alternatives for FBS
 
unfortunately most of those high nutrient media have a cruelty (or at least non-"vegetarian") aspect. I recall using a media containing calf brains


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