| | Re: Transwell Chemotaxis
I spent 7 years studying chemotaxis. In a word, transwells are shit. In fact, that's an insult to shit. The gradient they produce is non-physiological (upto 10,000X change in concentration over a cell-length, compared to 0.6-2% in the real world), cells can migrate through them without much of the machinery needed in vivo, and all sorts of chemotactic defects are missed by this assay.
Boyden chambers are essentially the same thing, btw.
There are a lot of better assays out there - under agarose assays, ibidi chemotaxis chambers, t-mazes, 3D collagen assays, micropipette assays, etc. Even dunn chambers are better than transwells.