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Transwell Chemotaxis

Transwell Chemotaxis - Cell Biology and Cell Culture

Transwell Chemotaxis - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 03-17-2011, 11:33 PM
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Default Transwell Chemotaxis



Hi, Im a final year biomed student currently doing my project.

I am looking into the migration of cells (THP-1) cells to chemoattractants using a disposable transwell plate.

I just wondered if anyone has had any experience in using the traswell plates as appose to the boyden chambers?

Any advise and help would be greatly appreciated.
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Old 03-18-2011, 04:26 PM
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Default Re: Transwell Chemotaxis

I spent 7 years studying chemotaxis. In a word, transwells are shit. In fact, that's an insult to shit. The gradient they produce is non-physiological (upto 10,000X change in concentration over a cell-length, compared to 0.6-2% in the real world), cells can migrate through them without much of the machinery needed in vivo, and all sorts of chemotactic defects are missed by this assay.

Boyden chambers are essentially the same thing, btw.

There are a lot of better assays out there - under agarose assays, ibidi chemotaxis chambers, t-mazes, 3D collagen assays, micropipette assays, etc. Even dunn chambers are better than transwells.

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Old 04-09-2011, 12:26 AM
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Default Re: Transwell Chemotaxis

Transwell inserts are OK. I believe 8 micrometer ones should work for THP-1. Use monocyte chemoattractant protein 1 as a positive control. Load a lot of cells and give it enough time to make sure more than a dozen cells migrate through.

Other systems like under agarose assays are great, but are more trouble than it's worth unless you are making a career out of studying chemotaxis.



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