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Need help culturing cancer and dendritic cells!

Need help culturing cancer and dendritic cells! - Cell Biology and Cell Culture

Need help culturing cancer and dendritic cells! - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 02-08-2011, 05:19 PM
Pipette Filler
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Default Need help culturing cancer and dendritic cells!



I'm trying to culture ovarian endometrioid carcinoma cell line TOV-112D (purchased as ATCC 11731) and monocyte/immature dendritic cell line JAWSII (purchased as ATCC 11904). I used a 1:1 mixture of medium 199 and MCDB 105 with PenStrep to culture the cancer cells, and a fully supplemented AMEM with PenStrep for the dendritic cells. After 2 weeks I noticed small fungal colony growth (but no bacterial) in all 10 flasks of the cancer cells, and lots of bacterial growth (but no fungal) in all 10 of the dendritic cell flasks.

Any suggestions? Please help!

Thanks, Jon
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cancer , cell culture , cells , culturing , dendritic , ovarian


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