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#1
08-05-2010, 05:32 PM
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 How do I grow my HepG2s? I have just received frozen HepG2 cells from ATCC and thawed them and they are (hopefully) growing away in the incubator, but they do not come with a recommended seeding density, nor a recommended subculturing density. Looking at published studies there are quite a range of seeding densities used, and no mention of when to split. Also, when do they reach confluence/full maturity? Thank you Tatiana   |
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#2
08-06-2010, 03:22 AM
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| Re: How do I grow my HepG2s? Hi Subculture at 50-70% confluency, otherwise they clump too much and hard to separate even with trypsinization. These can grow with sugar and water they are so hardy, but keep them happy with alpha-MEM or DMEM and 10% FBS. |
| The Following User Says Thank You to admin For This Useful Post: | ||
t.christides (08-08-2010)
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