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How do I grow my HepG2s?

How do I grow my HepG2s? - Cell Biology and Cell Culture

How do I grow my HepG2s? - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 08-05-2010, 05:32 PM
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Default How do I grow my HepG2s?



I have just received frozen HepG2 cells from ATCC and thawed them and they are (hopefully) growing away in the incubator, but they do not come with a recommended seeding density, nor a recommended subculturing density.
Looking at published studies there are quite a range of seeding densities used, and no mention of when to split.

Also, when do they reach confluence/full maturity?

Thank you

Tatiana
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Old 08-06-2010, 03:22 AM
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Default Re: How do I grow my HepG2s?

Hi Subculture at 50-70% confluency, otherwise they clump too much and hard to separate even with trypsinization.

These can grow with sugar and water they are so hardy, but keep them happy with alpha-MEM or DMEM and 10% FBS.
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