Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture
Register Search Today's Posts Mark Forums Read

Cell Biology and Cell Culture Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


low pH medium for HEK cells

low pH medium for HEK cells - Cell Biology and Cell Culture

low pH medium for HEK cells - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-17-2010, 09:37 AM
Pipette Filler
Points: 17, Level: 1 Points: 17, Level: 1 Points: 17, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2010
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default low pH medium for HEK cells



I'm still quite unexperienced in cell culture, so I hope you can help me.
I need to grow HEK cells at a relatively low pH (~6.0 - 6.5). This should kill my transfected cells, whereas normal HEKs should survive (at least for a few days). I normally grow them in RPMI medium (+ 10 % FCS + several other supplements). I tried lowering the pH to 6.0, but within 2 days (37C, 5% CO2) it rises to about 7.0. I think the medium is HEPES-buffered. Are there other media available, that are un buffered or MES-buffered? Or should I change CO2 %?
Thanks for your suggestions!
Reply With Quote
  #2  
Old 07-30-2010, 01:50 AM
Volunteer
Points: 1,873, Level: 26 Points: 1,873, Level: 26 Points: 1,873, Level: 26
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2009
Posts: 33
Thanks: 0
Thanked 4 Times in 3 Posts
Default Re: low pH medium for HEK cells

I am not sure whether CO2-independent medium (you can get it from Sigma) will be helpful or not. At least those medium won't be affect by CO2 (the pH should not change).

Or maybe you can put your cells in an incubator without CO2, or use flasks that have no filter in lids that wont' allow CO2 to enter your flasks. (for my understanding, CO2 is just for pH value, maybe I am wrong)
Reply With Quote
Reply

Tags
cells , hek , low , medium


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Characteristics of cancerous cells aftabac Cell Biology and Cell Culture 2 08-26-2011 11:40 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
How to isolate adult from circulation? peterish Stem Cell Forum 0 05-19-2008 10:02 PM
Cryopreservation of Lymphocytes pipette_man Cell Biology and Cell Culture 1 11-09-2007 05:05 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 04:56 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14753 seconds with 16 queries