I'm still quite unexperienced in cell culture, so I hope you can help me.
I need to grow HEK cells at a relatively low pH (~6.0 - 6.5). This should kill my transfected cells, whereas normal HEKs should survive (at least for a few days). I normally grow them in RPMI medium (+ 10 % FCS + several other supplements). I tried lowering the pH to 6.0, but within 2 days (37°C, 5% CO2) it rises to about 7.0. I think the medium is HEPES-buffered. Are there other media available, that are un buffered or MES-buffered? Or should I change CO2 %?
Thanks for your suggestions!