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#1
06-25-2010, 06:31 PM
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 Cell count in lysis buffer I've harvested some cells using a lysis buffer (APL buffer from the qiagen allprep protein / RNA kit). I added 150 ul to each well (24well plate) then stored cells + Apl buffer in -80 degree freezer , so the cells have only been subjected to the buffer briefly before freezing. I now want to do a cell count is this possible once un frozen or will the cells be destroyed already. Is there a possible solution? This same situation had also been done with the qiagen rtf buffer another lysis buffer. Help anyone you may be able to save me weeks of re culture  |
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| buffer , cell , count , lysis |
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