Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture
Register Search Today's Posts Mark Forums Read

Cell Biology and Cell Culture Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


Cell count in lysis buffer

Cell count in lysis buffer - Cell Biology and Cell Culture

Cell count in lysis buffer - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-25-2010, 05:40 PM
Pipette Filler
Points: 24, Level: 1 Points: 24, Level: 1 Points: 24, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2010
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Cell count in lysis buffer



I've harvested some cells using a lysis buffer (APL buffer from the qiagen allprep protein / RNA kit). I added 150 ul to each well (24well plate) then stored cells + Apl buffer in -80 degree freezer , so the cells have only been subjected to the buffer briefly before freezing. I now want to do a cell count is this possible once un frozen or will the cells be destroyed already. Is there a possible solution?

This same situation had also been done with the qiagen rtf buffer another lysis buffer.

Help anyone you may be able to save me weeks of re culture
Reply With Quote
Reply

Tags
buffer , cell , count , lysis


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Cell count in lysis buffer Caddie Cell Biology and Cell Culture 0 06-25-2010 06:31 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
A Human Cytome Project - an idea - Update 14 March 2005 Peter Van Osta Cell Biology and Cell Culture 0 03-14-2005 02:27 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 01:43 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16340 seconds with 16 queries