Cell count in lysis buffer
I've harvested some cells using a lysis buffer (APL buffer from the qiagen allprep protein / RNA kit). I added 150 ul to each well (24well plate) then stored cells + Apl buffer in -80 degree freezer , so the cells have only been subjected to the buffer briefly before freezing. I now want to do a cell count is this possible once un frozen or will the cells be destroyed already. Is there a possible solution?
This same situation had also been done with the qiagen rtf buffer another lysis buffer.
Help anyone you may be able to save me weeks of re culture
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