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-   -   Cell count in lysis buffer (http://www.molecularstation.com/forum/cell-biology-cell-culture/73228-cell-count-lysis-buffer.html)

Caddie 06-25-2010 05:40 PM

Cell count in lysis buffer
 
I've harvested some cells using a lysis buffer (APL buffer from the qiagen allprep protein / RNA kit). I added 150 ul to each well (24well plate) then stored cells + Apl buffer in -80 degree freezer , so the cells have only been subjected to the buffer briefly before freezing. I now want to do a cell count is this possible once un frozen or will the cells be destroyed already. Is there a possible solution?

This same situation had also been done with the qiagen rtf buffer another lysis buffer.

Help anyone you may be able to save me weeks of re culture


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