i think this is the second time i tried posting this- there seems to be a problem with the last time i tried.
uh, ive been trying to culture hepg2 cells for my thesis, but they dont seem to adhere very much, and the floating cells seems to grow, and then eventually die. i thaw them and add 10% FBS in MEM dropwise and replace the media with DMSO a few days later, and they still die. please help. i don't put any pen/strep and am under the assumption that these hepg2 cells should be easy enough to culture, but damn they form aggregates/clumps and just float. the attached cells dont seem to multiply much. in some of my trials the floating cells multipled after a few days, but then almost instantaneously die, without the media changing colour.
i tried tranferring the floating cells to another flask, but the previous flask's cells died too.
any advice on how to properly grow the cells? im in dire need of assistance
please please please help.