|Register||Search||Today's Posts||Mark Forums Read|
|Cell Biology and Cell Culture Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.|
| ||LinkBack||Thread Tools||Display Modes|
Attach cells fails to change their morphology after Trypsinization
In Trpsinization of Mesenchymal stem cells i am using 0.05% Trypsin with 0.53mM EDTA but i noticed that they take very long for deatachment 20 to 25 min and after trypsinization most of the cells fails to reattach to the palstic surface or if they attach they didnt change thier morphology they remains round so next time i diluted the Trypsin and minimiza the duration of incubation but i got the same results.I use Ca & Mg free PBS for washing of cells and after trypsinization i add complete medium with 10% FBS to stop the action of trypsin.
i fail to sort out the problem .Can anyone give me some suggestion.
|attach , cells , change , fails , morphology , trypsinization|
|Thread||Thread Starter||Forum||Replies||Last Post|
|mouse hepatocytes isolation||amicia||Cell Biology and Cell Culture||39||05-11-2012 11:52 AM|
|Characteristics of cancerous cells||aftabac||Cell Biology and Cell Culture||2||08-26-2011 11:40 PM|
|Human Cytome Project - Update 24 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||1||08-01-2010 02:18 PM|
|Attach cells fails to change their morphology after Trypsinization||Kanwal||Stem Cell Forum||2||04-07-2010 11:22 AM|
|Human Cytome Project - Update 6 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||0||01-06-2005 10:18 AM|