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| Cell Biology and Cell Culture Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology. |
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#1
04-01-2010, 07:30 PM
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 my frezee was unfrezzed and all cells die Ineed help last week realized that -80 was unfreezed and I start to check my cell stock and most all these die. So I would like if I could do something to rescue at last some of them?  |
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#2
01-28-2011, 07:49 PM
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| Re: my frezee was unfrezzed and all cells die Don't you store your frozen cells inside in a nitrogen tank? That's the way we do it here and even then is not 100% guaranteed that all the cells would survive. If your cells could make it from what you describe, they should be damaged at some degree. I wouldn't dare use them for further experiments. I highly recommend you try to get new ones. |
| The Following User Says Thank You to luisillo For This Useful Post: | ||
admin (01-28-2011)
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| cells , die , frezee , unfrezzed |
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