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Cell Mortality: Please Help me

Cell Mortality: Please Help me - Cell Biology and Cell Culture

Cell Mortality: Please Help me - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 12-25-2009, 02:02 PM
Pipette Filler
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Red face Cell Mortality: Please Help me



Dear all,

I haves a big problem for my cell culture recently .
I'm culturing the adherent mouse macrophage cell line Raw264.7 for 2 years and it goe's well until now.
The problems i'm facing now is poor cell morphology and viability They are adherent as they are supposed to be, but we saw a lot of mortality, a lot of vacuoles in cells and many lysis areas with large size cells (that seems apoptototic). I haven't any bacterial or fungal contamination that can be visually observed under the microscope.
I have used new: RPMI(SIGMA), FBS(Hyclone), Tissu culture flask and i didin't seen any improvement.
I wonder whether there is any perceptible signs for mycoplasma contamination or another type of contamination that I can use to judge my case?
It drive me crazy !!!
All comments are welcom.

Many thanks.
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  #2  
Old 12-29-2009, 12:28 PM
Pipette Filler
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Default Re: Cell Mortality: Please Help me

No reply to my post.
I really need your help so please help me.

Many thanks
Merry christmas and happy new year to all.
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  #3  
Old 02-22-2012, 07:53 PM
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Default Re: Cell Mortality: Please Help me

thanks
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