In the last days we have been discussing a very trigger question in our lab about cells counting. We realize that most of our co-workers didn't know how to count cells.
What is your opinion?
We have a cell culture with 20mL.
We pick up 1mL to and eppendorf and ressuspend.
From that we pick up 10uL to a new epp and then we add 90ul of tripan blue.
From this last mixture, we pick up 10uL to a hemacytometer counting chamber.
We count something an average of 58 cells in 1/16 squares.
Question: what is the final concentration p/ mL?